Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Jesse D Woodson; Matthew S Joens; Andrew B Sinson; Jonathan Gilkerson; Patrice A Salomé; Detlef Weigel; James A Fitzpatrick; Joanne Chory

doi:10.1126/science.aac7444

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Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

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Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts

Jesse D Woodson, Matthew S Joens, Andrew B Sinson, Jonathan Gilkerson, Patrice A Salomé, Detlef Weigel, James A Fitzpatrick and Joanne Chory

Science (American Association for the Advancement of Science), Vol.350(6259), pp.450-454

10/23/2015

DOI: https://doi.org/10.1126/science.aac7444

Handle:

https://hdl.handle.net/20.500.12741/rep:5691

PMCID: PMC4863637

PMID: 26494759

Abstract

Arabidopsis Proteins - genetics

Oxidative Stress

Stress, Physiological - genetics

Ubiquitin - metabolism

Ubiquitin-Protein Ligases - metabolism

Arabidopsis - ultrastructure

Mitochondria - metabolism

Chloroplasts - metabolism

Arabidopsis - metabolism

Arabidopsis - genetics

Arabidopsis Proteins - metabolism

Genes, Lethal

Singlet Oxygen - metabolism

Ubiquitination

Metabolic Networks and Pathways

Adaptation, Physiological - genetics

Mutation

Photosynthesis

Ubiquitin-Protein Ligases - genetics

Energy production by chloroplasts and mitochondria causes constant oxidative damage. A functioning photosynthetic cell requires quality-control mechanisms to turn over and degrade chloroplasts damaged by reactive oxygen species (ROS). Here, we generated a conditionally lethal Arabidopsis mutant that accumulated excess protoporphyrin IX in the chloroplast and produced singlet oxygen. Damaged chloroplasts were subsequently ubiquitinated and selectively degraded. A genetic screen identified the plant U-box 4 (PUB4) E3 ubiquitin ligase as being necessary for this process. pub4-6 mutants had defects in stress adaptation and longevity. Thus, we have identified a signal that leads to the targeted removal of ROS-overproducing chloroplasts.

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Title: Ubiquitin facilitates a quality-control pathway that removes damaged chloroplasts
Creators: Jesse D Woodson - Plant Biology Laboratory, The Salk Institute, La Jolla, CA
Matthew S Joens - Waitt Advanced Biophotonics Center, The Salk Institute, La Jolla, CA
Andrew B Sinson - Plant Biology Laboratory, The Salk Institute, La Jolla, CA. Division of Biological Sciences, University of California-San Diego, La Jolla, CA
Jonathan Gilkerson - Plant Biology Laboratory, The Salk Institute, La Jolla, CA. Howard Hughes Medical Institute, The Salk Institute, La Jolla, CA
Patrice A Salomé - Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany
Detlef Weigel - Department of Molecular Biology, Max Planck Institute for Developmental Biology, Tübingen, Germany
James A Fitzpatrick - Waitt Advanced Biophotonics Center, The Salk Institute, La Jolla, CA
Joanne Chory - Plant Biology Laboratory, The Salk Institute, La Jolla, CA. Howard Hughes Medical Institute, The Salk Institute, La Jolla, CA. chory@salk.edu
Academic Unit: Department of Biological Sciences
Publisher: United States
Publication Details: 10/23/2015
Grant note: P30 CA014195 / NCI NIH HHS F32 GM096610 / NIGMS NIH HHS 1F32GM096610 / NIGMS NIH HHS Howard Hughes Medical Institute P30 NS072031 / NINDS NIH HHS
Identifiers: 99257861851801671; https://hdl.handle.net/20.500.12741/rep:5691; https://doi.org/10.1126/science.aac7444
Language: English