Abstract
A microscale capillary electrophoresis (CE) system was created with conventional devices. Mechanical and thermal techniques were implemented in the creation of a microchip in a microscope glass slide for analyte separation. The detection system consisted of a high-intensity LED used as light source and a photodiode, with an amplifier integrated in the same chip, as the detector. LabVIEW was used for analysis and acquisition of data from the system in which two commercial dyes, Evans Blue and Night Blue, were separated. The performance of the microsystem was compared to a conventional CE instrument. It was found that although the microscale CE has a relatively low sensitivity, it compensates by providing better resolution and shorter time for analysis and low cost.