Abstract
A lytic bacteriophage RG-2014 infecting a biofilm forming multidrug resistant bacterium
Delftia tsuruhatensis
strain ARB-1 as its host was isolated from a full-scale municipal wastewater treatment plant. Lytic phage RG-2014 was isolated for developing phage based therapeutic approaches against
Delftia tsuruhatensis
strain ARB-1. The strain ARB-1 belongs to the
Comamonadaceae
family of the
Betaproteobacteria
class. RG-2014 was characterized for its type, burst size, latent and eclipse time periods of 150 ± 9 PFU/cell, 10-min, <5-min, respectively. The phage was found to be a dsDNA virus belonging to the
Podoviridae
family. It has an isometric icosahedrally shaped capsid with a diameter of 85 nm. The complete genome of the isolated phage was sequenced and determined to be 73.8 kbp in length with a G + C content of 59.9%. Significant similarities in gene homology and order were observed between
Delftia
phage RG-2014 and the
E. coli
phage N4 indicating that it is a member of the N4-like phage group.