Abstract
Over the course of many years of antibiotic use, there has been the development of resistance to antibiotics in many human pathogens, creating the current antibiotic resistance crisis. Scientists have been searching for microorganisms with antibiotic activity against the clinically relevant group of bacteria, known as ESKAPE pathogens. These Pathogens are Escherichia Coli, Staphylococus Aureus, Klebsiella Pnemoniae, Acenetobater Baumanii, Psudomonas Putida and Entrobacter. A Microorganism’s ability to kill the ESKAPE pathogens are determined by the secondary metabolites release outside of the bacterial cell. These are natural products with various chemical structures and maintain biological activity. In present study, active metabolites were extracted from bacterial strains that were collected by student researchers in the Diversity of Microorganism course at Sacramento state University. Bioactive compounds were extracted by utilizing solvent extraction method and dried for future analysis. We developed and proposed multiple methods to identify the antibiotic activity of the extracted secondary metabolites. We anticipation to isolate, purify, and identify the bioactive compounds of interest and evaluate their role in antibacterial activity.